Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm
نویسندگان
چکیده
منابع مشابه
A Binding Mode Hypothesis of Tiagabine Confirms Liothyronine Effect on γ-Aminobutyric Acid Transporter 1 (GAT1)
Elevating GABA levels in the synaptic cleft by inhibiting its reuptake carrier GAT1 is an established approach for the treatment of CNS disorders like epilepsy. With the increasing availability of crystal structures of transmembrane transporters, structure-based approaches to elucidate the molecular basis of ligand-transporter interaction also become feasible. Experimental data guided docking o...
متن کاملGATMD: γ-Aminobutyric Acid Transporter Mutagenesis Database
Since the cloning of the first γ-aminobutyric acid (GABA) transporter (GAT1; SLC6A1) from rat brain in 1990, more than 50 published studies have provided structure-function information on investigator-designed rat and mouse GAT1 mutants. To date, more than 200 of 599 GAT1 residues have been subjected to mutagenesis experiments by substitution with different amino acids, and the resulting transp...
متن کاملAn Intermediate State of the γ-Aminobutyric Acid Transporter Gat1 Revealed by Simultaneous Voltage Clamp and Fluorescence
The rat gamma-aminobutyric acid transporter GAT1 expressed in Xenopus oocytes was labeled at Cys74, and at one or more other sites, by tetramethylrhodamine-5-maleimide, without significantly altering GAT1 function. Voltage-jump relaxation analysis showed that fluorescence increased slightly and monotonically with hyperpolarization; the fluorescence at -140 mV was approximately 0. 8% greater tha...
متن کاملTwo Discontinuous Segments in the Carboxyl Terminus Are Required for Membrane Targeting of the Rat -Aminobutyric Acid Transporter-1 (GAT1)*
Like all members of the Na /Cl -dependent neurotransmitter transporter family, the rat -aminobutyric acid transporter-1 (GAT1) is sorted and targeted to specialized domains of the cell surface. Here we identify two discontinuous signals in the carboxyl terminus of GAT1 that cooperate to drive surface expression. This conclusion is based on the following observations. Upon deletion of the last 3...
متن کاملIdentification and characterization of γ-aminobutyric acid uptake system
State Key Laboratory of Microbial Resources and Dept. of Industrial 4 Microbiology and Biotechnology, Institute of Microbiology, Chinese Academy of 5 Sciences, Beijing 100101, PR China; Key Laboratory of Agricultural and 6 Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of 7 Sciences, Wuhan 430071, People's Republic of China 8 9 Running title 10 GABA uptake system in C....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Research
سال: 2000
ISSN: 1001-0602,1748-7838
DOI: 10.1038/sj.cr.7290035